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    hPDL-1/mPDL-1null

    1. 基本信息

    品系名称:B6-Tg(CAG- hPDL-1)5Vst mPDL-1em1Vst/Vst

    常用名mPDL-1null PD-1人源化小鼠; hPD-1/mPDL-1null小鼠

    背景C57BL/6NCrl

    毛色黑色


    品系建立

    采用转基因的方法,构建CAG- hPDL-1表达载体,酶切分离表达载体片段,将该片段注射到C57BL/6NCrl小鼠的原核中。在后代中筛选到合适表达量的阳性小鼠,与mPD-L1null小鼠交配,获得同时敲除mPDL-1和过表达hPDL-1的小鼠。


    2. 应用领域

    -人源化和癌症治疗

    -免疫治疗

    -药物筛选


    3. 参考文献

    (1)Association of PD-L1 expression and PD-L1 gene polymorphism with poor prognosis in lung adenocarcinoma and squamous cell carcinoma.Yeo MK, Choi SY, Seong IO, Suh KS, Kim JM, Kim KH.Hum Pathol. 2017 Oct; 68: 103-111. doi: 10.1016/j.humpath.2017.08.016. Epub 2017 Aug 26.

    (2)Gene code CD274/PD-L1: from molecular basis toward cancer immunotherapy.Fabrizio FP, Trombetta D, Rossi A, Sparaneo A, Castellana S, Muscarella LA.Ther Adv Med Oncol. 2018 Dec 17;10:1758835918815598. doi: 10.1177/1758835918815598. eCollection 2018.